kchip2 (Alomone Labs)

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Alomone Labs kchip2
Kchip2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kchip2/Anti-KChIP2+Antibody/pm41490034-185-39-41
Average 94 stars, based on 11 article reviews

kchip2 - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: Glucagon-Like Peptide-1 Protects Against Atrial Fibrillation and Atrial Remodeling in Type 2 Diabetic Mice
Article Snippet: The membrane was blocked for 1 hour at room temperature with 1% casein in Tris-buffered saline (TBS; Bio-Rad Laboratories). .. Blots were incubated overnight at 4 °C with rabbit-raised primary antibodies to Kv4.2 (1:500, Alomone Labs), Kv4.3 (1:1,000, Alomone Labs), Kv1.5 (1:500, Alomone Labs) or KChIP2 (1:500, Alomone Labs). .. The Western blot membranes were then washed 3 times for 10 min in 1% TBST (TBS with 1% Tween 20 (Bio-Rad Laboratories)) before secondary antibody binding.

Blocking Assay:

Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice.
Article Snippet: After transfer, membranes were blocked with 5% skim milk (Solarbio, China) in TBST (Solarbio, China) for 2 h at room temperature. .. Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; InvitrogenTM, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA). .. Membranes were washed three times with TBST (10 min each) and then incubated for1 h at room temperature with HRP-conjugated goat anti-rabbit IgG (1:10,000; Solarbio, China) for detection of rabbit primary antibodies.

Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice
Article Snippet: After transfer, membranes were blocked with 5% skim milk (Solarbio, China) in TBST (Solarbio, China) for 2 h at room temperature. .. Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; InvitrogenTM, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA). .. Membranes were washed three times with TBST (10 min each) and then incubated for1 h at room temperature with HRP-conjugated goat anti-rabbit IgG (1:10,000; Solarbio, China) for detection of rabbit primary antibodies.

Western Blot:

Article Title: Intravenous Semaphorin 3A Administration Maintains Cardiac Contractility and Improves Electrical Remodeling in a Mouse Model of Isoproterenol-Induced Heart Failure.
Article Snippet: .. The samples were subjected to Western blotting with antibodies against Kv4.3 (#APC-017; Alomone Labs, Inc., Jerusalem, Israel), KChIP2 (#APC-142; Alomone Labs), sarcoendoplasmic reticulum Ca2+-ATPase 2 (SERCA2; #ab3625; Abcam, Cambridge, UK), and GAPDH (#GTX28245; GeneTexR, Inc., Irvine, CA, USA). .. Rabbit IgG HRP-conjugated Whole Ab (#Cytiva NA934; Sigma) was used as the secondary antibody.

Control:

Article Title: Knockout of interleukin-17A diminishes ventricular arrhythmia susceptibility in diabetic mice via inhibiting NF-κB-mediated electrical remodeling.
Article Snippet: Interleukin-17A (IL-17), a potent proinflammatory cytokine, has been shown to participate in cardiac electrical disorders.. Diabetes mellitus is an independent risk factor for ventricular arrhythmia.. In this study, we investigated the role of IL-17 in ventricular arrhythmia of diabetic mice.

Review